Firepol polymerase

Description

FIREPol^® is a highly processive, thermostable DNA Polymerase. Due to its genetic modifications, it has enhanced stability at room temperature with no activity loss for up to 1 month.

Recommended for routine applications (fragment up to 3 kb from genomic DNA).

Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning.

The fidelity of FIREPol^® is similar to a regular Taq DNA Polymerase (error rate per nucleotide ca 2.5 x10^-5).

Properties

Concentration: 5 U/µl

Error rate per nucleotide per cycle is app. 2.5 x 10^-5

Accuracy is app. 4 x 10⁴

Estimated half life at 95ºC is 1.5 hours.

Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH

Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.

Kit Components

FIREPol^® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers.

FIREPol^® 10x Buffer B (without Mg²⁺ ): 0.8 M Tris-HCl, 0.2 M (NH₄)₂SO₄, 0.2% w/v Tween-20.

FIREPol^® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency.

FIREPol^® 10x Buffer BD (without Mg²⁺ and detergent): 0.8 M Tris-HCl, 0.2 M (NH₄)₂SO₄.

25 mM MgCl₂

GC-rich Enhancer is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates).