Transforming the art of cell culture into scientific engineering.
Cell cultures have been used since the 1930’s. Today, in the 21st century, cell cultures are used in laboratories for biological research, as factories for production of engineered molecules, and as keystones in tissue repair and renewal. Today’s profound knowledge of cell biology allows researchers to better comprehend genetics and the foundations of life. This knowledge base, and the efficiency of cell culture, using cell culture CelartiaTM products will yield tremendous gains in medicine, biotechnology and biology.
The CelartiaTM cell culture system, based on Petaka G3, applies scientific reasoning and sound engineering to transform the art of cell culture into a science of standardized results.
Using an in-depth knowledge of research methodology and an understanding of the industrial and medical need for cell cultures, Celartia TM scientists focus on cell biology, specifically, the creation of cutting-edges tool and procedures for growing cells in the most scientific, consistent and efficient manner.
PetakaG3 basic concepts
Dimensions:
Inches: 5.03 x 3.38 x 0.21
mm: 127.8 x 85.7 x 5.45
Maximum media volume: 25 mL
Maximum cell culture surface: 150 cm2
Characterization:
The cell culture device PetakaG3 is an innovative bioreactor designed for mammalian cell culture in physiologic tissue conditions.
The distinctive feature of PetakaG3 is that being a disposable device incorporates an automatic gas diffusion control system (AGD-CS) that works interactively with the cells growing and living inside, WITHOUT HYPOXIA CHAMBERS, GLOVE BOXES OR THREE-GAS INCUBATORS.
For cells cultured in PetakaG3 and incubated in regular 21% oxygen atmosphere the AGD-CS provides:
1) Media dissolved oxygen concentration within the limits of the physiologic oxygen tensions in physiologic tissues (from 75 mmHg down to 15 mmHg) monitorable with a dissolved oxygen fluorescent sensor dot. Because this advanced trait PetakaG3 is the safest, smallest, unique portable disposable hypoxia chamber, also available with integrated DO sensor.
2) Media dissolved CO2 concentration, enough to maintain a physiologic pH without external extra-supply of carbon dioxide gas.
3) Controlled media evaporation even at 2% to 10% atmospheric Relative Humidity (RH).
PetakaG3 is available in several options for culturing all kinds of cells from vertebrate and invertebrate organisms, especially suited for stem cells, primary cultures and tumor cells. PetakaG3 LOT & PetakaG3 HOT with hydrophilic surface developed for anchored dependent cells growth and PetakaG3 FLAT with a hydrophobic surface, not permissive of cell adhesion, for non adherent cells growth and 3D cellular clusters and aggregates. PetakaG3-Matrix is available carrying different surface molecular matrices, such as Collagen, Vitronectin, Matrigel, etc. and also structural matrices (nano-fibers mesh), or combinations of structural and molecular.
4) PetakaG3 allows cell cultures of two different cell types in the same device. Both cell layers are separated by 3mm of media, which is appropriate to study intercellular interactions through diffusible factors and exosome exchange. PetakaG3 is ideal for transfection of both cell layers with two different gene constructs in the same device with negligible cross transfection
PetakaG3 2% Oxygen (Autonomous hypoxia chamber for cell culture)
Inside Celartia’s hypoxia chambers cells are exposed to a progressive reduction of available oxygen, from 45 mmHg partial pressure down to 13.32 mmHg, the lowest oxygen tensions recorded on living vertebrates: .
Outstandingly, Celartia’s Petaka G3 hypoxia chambers achieve this in open atmosphere, inside a regular incubator, without accessory gas canisters or electrical power, electronic sensors or feedback electronics.
Celartia’s hypoxia chamber’s use is even easier than regular multi-well plates, and does not need special training to use them at top level of efficiency.
