Plant genomic DNA
FIREPol PCR Master Mix for routine applications 2.5 MgCl2
€28.01
In stock
SKU
SB_04-11-00125
For research use only.
PCR Master Mix for routine applications
- suitable for templates up to 5 kb
- reaction set-up and shipment without dry ice
- all-in-one master mix format reduces pipetting errors and saves time
- amplicons are suitable for Sanger sequencing post-PCR
Description
Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water.
We recommend using FIREPol® Master Mix without ready to load feature in all PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications.
Properties
Concentration: 5x
Hot-start: no
Ready to load: no
Fidelity: same as Taq
Cloning Type: T/A Cloning
Amplicon Size: up to 5 kb
Mix Components
FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase
5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
| Product Data Sheet | https://solisbiodyne.com/pics/9751_DS-04-11-125_v2_FIREPol_Master_Mix_with_12.5_mM_MgCl2_revised_18.10.2021.pdf_Kit_revised_02.04.2024.pdf |
|---|---|
| Shipping Temp | Ambient |
| Storage Temp | -20°C |
| Shelf life | 2 years |
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